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School of the Biological Sciences

 

 

Principal Investigator, Department of Physiology, Development and Neuroscience

Thorsten Boroviak aims to illuminate the cell-fate decision of early primate development by transcriptional and epigenetic profiling of marmoset embryos. Learn more. 

 

Research Areas


Embryogenesis
Pluripotency
Extraembryonic stem cells
Gastrulation
 
 

Techniques


Transcriptional and epigenetic profiling
Combined single-cell RNA-seq
Bisulfite sequencing
Computational analysis
 
 
   

 

Research Theme


   Reproduction, Development and Lifelong Health

 

Publications

Journal articles

2022

  • Boroviak, TE., 2022. A human embryo model cracks symmetry breaking. Cell Stem Cell, v. 29
    Doi: http://doi.org/10.1016/j.stem.2022.05.009
  • 2021

  • Betto, RM., Diamante, L., Perrera, V., Audano, M., Rapelli, S., Lauria, A., Incarnato, D., Arboit, M., Pedretti, S., Rigoni, G., Guerineau, V., Touboul, D., Stirparo, GG., Lohoff, T., Boroviak, T., Grumati, P., Soriano, ME., Nichols, J., Mitro, N., Oliviero, S. and Martello, G., 2021. Metabolic control of DNA methylation in naive pluripotent cells. Nat Genet, v. 53
    Doi: http://doi.org/10.1038/s41588-020-00770-2
  • Bergmann, S., Schindler, M., Munger, C., Penfold, CA. and Boroviak, TE., 2021. Building a stem cell-based primate uterus. Commun Biol, v. 4
    Doi: http://doi.org/10.1038/s42003-021-02233-8
  • 2020

  • Ross, C. and Boroviak, TE., 2020. Origin and function of the yolk sac in primate embryogenesis. Nat Commun, v. 11
    Doi: http://doi.org/10.1038/s41467-020-17575-w
  • Stirparo, GG., Kurowski, A., Strawbridge, SE., Stuart, H., Boroviak, TE., Yanagida, A. and Nichols, J., 2020. Oct4 regulates embryonic pluripotency via metabolic mechanisms and Stat3 signalling
    Doi: 10.1101/2020.01.28.922856
  • 2018

  • Stirparo, GG., Boroviak, T., Guo, G., Nichols, J., Smith, A. and Bertone, P., 2018. Correction: Integrated analysis of single-cell embryo data yields a unified transcriptome signature for the human pre-implantation epiblast (doi: 10.1242/dev.158501). Development, v. 145
    Doi: http://doi.org/10.1242/dev.169672
  • 2017

  • Boroviak, T. and Nichols, J., 2017. Primate embryogenesis predicts the hallmarks of human naïve pluripotency Development, v. 144
    Doi: http://doi.org/10.1242/dev.145177
  • 2016

  • Scognamiglio, R., Cabezas-Wallscheid, N., Thier, MC., Altamura, S., Reyes, A., Prendergast, Á., Baumgärtner, D., Carnevalli, LS., Atzberger, A., Haas, S., von Paleske, L., Boroviak, T., Wörsdörfer, P., Essers, MA., Kloz, U., Eisenman, RN., Edenhofer, F., Bertone, P., Huber, W., van der Hoeven, F., Smith, A. and Trumpp, A., 2016. Myc Depletion Induces a Pluripotent Dormant State Mimicking Diapause. Cell, v. 164
    Doi: http://doi.org/10.1016/j.cell.2015.12.033
  • Scognamiglio, R., Cabezas-Wallscheid, N., Thier, MC., Altamura, S., Reyes, A., Prendergast, Á., Baumgärtner, D., Carnevalli, LS., Atzberger, A., Haas, S., von Paleske, L., Boroviak, T., Wörsdörfer, P., Essers, MA., Kloz, U., Eisenman, RN., Edenhofer, F., Bertone, P., Huber, W., van der Hoeven, F., Smith, A. and Trumpp, A., 2016. Myc depletion induces a pluripotent dormant state mimicking diapause Cell, v. 164
    Doi: http://doi.org/10.1016/j.cell.2015.12.033
  • 2015

  • Nichols, J. and Boroviak, T., 2015. Maximizing Clonal Embryonic Stem Cell Derivation by ERK Pathway Inhibition. Methods Mol Biol,
    Doi: http://doi.org/10.1007/7651_2015_253
  • Boroviak, T., Loos, R., Lombard, P., Okahara, J., Behr, R., Sasaki, E., Nichols, J., Smith, A. and Bertone, P., 2015. Lineage-Specific Profiling Delineates the Emergence and Progression of Naive Pluripotency in Mammalian Embryogenesis. Dev Cell, v. 35
    Doi: http://doi.org/10.1016/j.devcel.2015.10.011
  • 2014

  • Boroviak, T. and Nichols, J., 2014. The birth of embryonic pluripotency. Philos Trans R Soc Lond B Biol Sci, v. 369
    Doi: http://doi.org/10.1098/rstb.2013.0541
  • Boroviak, T., Loos, R., Bertone, P., Smith, A. and Nichols, J., 2014. The ability of inner-cell-mass cells to self-renew as embryonic stem cells is acquired following epiblast specification. Nat Cell Biol, v. 16
    Doi: http://doi.org/10.1038/ncb2965
  • 2013

  • Tatsumoto, S., Adati, N., Tohtoki, Y., Sakaki, Y., Boroviak, T., Habu, S., Okano, H., Suemizu, H., Sasaki, E. and Satake, M., 2013. Development and characterization of cDNA resources for the common marmoset: one of the experimental primate models. DNA Res, v. 20
    Doi: http://doi.org/10.1093/dnares/dst007
  • 2011

  • Boroviak, T. and Rashbass, P., 2011. The apical polarity determinant Crumbs 2 is a novel regulator of ESC-derived neural progenitors. Stem Cells, v. 29
    Doi: http://doi.org/10.1002/stem.567
  • 2008

  • Reitinger, S., Boroviak, T., Laschober, GT., Fehrer, C., Müllegger, J., Lindner, H. and Lepperdinger, G., 2008. High-yield recombinant expression of the extremophile enzyme, bee hyaluronidase in Pichia pastoris. Protein Expr Purif, v. 57
    Doi: http://doi.org/10.1016/j.pep.2007.10.001
  • Stirparo, G., Kurowski, A., Yanagida, A., Bates, L., Strawbridge, S., Hladkou, S., Stuart, H., Boroviak, T., Rebelo Da Silva, J. and Nichols, J., Oct4 induces embryonic pluripotency via Stat3 signalling and metabolic mechanisms PNAS,
  • Boroviak, T., Hollfelder, F., Schindler, M., Siriwardena, D., Kohler, T., Ellermann, A., Slatery, E. and Munger, C., Agarose microgel culture delineates lumenogenesis in naïve and primed human pluripotent stem cells Stem Cell Reports,
  • Bergmann, S., Penfold, C., Slatery, E., Siriwardena, D., Drummer, C., Clark, S., Strawbridge, S., Kishimoto, K., Vickers, A., Tewary, M., Kohler, T., Hollfelder, F., Reik, W., Sasaki, E., Behr, R. and Boroviak, T., Spatial profiling of early primate gastrulation in utero Nature,
  • Malkowska, A., Penfold, C., Bergmann, S. and Boroviak, T., A hexa-species transcriptome atlas of mammalian embryogenesis delineates metabolic regulation across three different implantation modes Nature Communications,
    Doi: 10.1038/s41467-022-30194-x
  • Boroviak, T., Chen, Y., Siriwardena, D., Pavlinek, A. and Penfold, CA., An integrated atlas of human placental development delineates essential regulators of trophoblast stem cells Development,
  • Kohler, T., De Jonghe, J., Ellermann, A., Yanagida, A., Yanagida, A., Herger, M., Slatery, E., Weberling, A., Munger, C., Fischer, K., Mulas, C., Winkel, A., Ross, C., Bergmann, S., Franze, K., Chalut, K., Nichols, J., Boroviak, T. and Hollfelder, F., Plakoglobin is a mechanoresponsive regulator of naïve pluripotency Nature Communications,
  • Bertone, PN., Stirparo, G., Boroviak, T., Guo, G., Nichols, J. and Smith, A., Integrated analysis of single-cell embryo data yields a unified transcriptome signature for the human preimplantation epiblast Development,
  • Internet publications

    2018

  • Boroviak, T., Stirparo, G., Dietmann, S., Hernando-Herraez, I., Mohammed, H., Reik, W., Smith, A., Sasaki, E., Nichols, J. and Bertone, P., 2018. Single-cell transcriptome analysis of human, marmoset and mouse embryos reveals common and divergent features of preimplantation development
    Doi: 10.1101/385815