Genomics
DNA Sequencing is an essential tool in all areas of the Biological Sciences, supporting translational research, advances in disease diagnostics and treatment. Researchers use DNA sequencing to understand genetics variations and mutations that may play a role in development and disease.
Genomics Facilities across the School of Biological Sciences provide high quality DNA sequencing services using a variety of equipment and techniques. Our staff also offer support throughout experiments, from project design to library preparation, sequencing and analysis.
School-based Facilities open to all include:
- Genomics Facility at the Department of Biochemistry
- Genomics Facility at the Wellcome/Cancer Research UK Gurdon Institute
- Cambridge Genomic Services at the Department of Pathology
- Genomics Facility at the Wellcome - MRC Cambridge Stem Cell Institute
Members of the School can also access the Pre-clinical genome editing facility at the Cancer Research UK Cambridge Institute who provide genome editing technologies, including CRISPR.
Genomics Facility at the Department of Biochemistry
Equipment available in the facility:
- Applied Biosystems 3730XL
- Applied Biosystems 3130XL
- Agilent Bioanalyzer
- Covaris S220
- Illumina MiSeq
- Illumina NextSeq
- Oxford Nanopore Mk1C
- Sage Science Blue Pippin
Location
The facility is based in the Department of Biochemistry (see map)
Contact
Facility Manager: Shilo Titus
Email: Sequencing Team
Access
The DNA Sequencing facility is open to all members of the University.
Genomics Facility at the Wellcome/Cancer Research UK Gurdon Institute
Sequencing equipment and techniques available in the facility:
- Illumina NovaSeq 6000
Location
The facility is based in the Gurdon Institute (see map)
Contact
Academic Lead: Charles Bradshaw
Facility Technician Kay Harnish
Email: sequencing@gurdon.cam.ac.uk
Access
The facility is open to all members of the University and external members.
Cambridge Genomic Services at the Department of Pathology
Sequencing equipment and techniques available in the facility:
- Illumina NextSeq 500
- Illumina Miseq
- Access to NextSeq 2000
- Chromium Controller (10X single-cell sequencing)
- Oxford Nanopore
- Agilent Tapestation and Agilent Bioanalyser
- Covaris
- Affymetrix Microarray platform
- Illumina Microarray platform
- Bioinformatic analysis
Location
The facility is based in the Department of Pathology (see map)
Contact
Academic Lead: Anton Enright
Facility Managers: Alexandra Karcanias and Julien Bauer
Email: cgs@path.cam.ac.uk
Access
The is open to all members of the University and external users.
Genomics Facility at the Wellcome - MRC Cambridge Stem Cell Institute
Services available in the facility:
- Project planning and advice about your NGS project
- Covaris E220 shearing of DNA/RNA samples; chromatin samples
- Sample QC
- Library preparation and QC
- Bespoke projects will be considered
- Advice on choice of sequencing platform
- Close interaction with Bioinformatics support facility
The sequencing itself is not done in-house, but at CIGC in Cambridge which allows access to the latest Illumina sequencing technology.
Location
The facility is based in the Cambridge Stem Cell Institute (see map)
Contact
Genomics Facility Manager: Maike Paramor
Email: ngs-core@jcbc.cam.ac.uk
Access
The facility is open to all members of the University and external users from the academic and private sectors.