Flow cytometry is a powerful tool that provides rapid analysis of cells in solution through optical signalling and light detection. Flow cytometry is most widely used in mammalian biology, however the technique can be applied to almost any particle with useful optical properties including bacteria, viruses, subcellular organelles, and artificial particles, for example, polystyrene beads bearing ligands or antibodies.
Flow cytometry has applications in immunology, molecular biology, bacteriology, virology, cancer biology and infectious disease monitoring.
School-based Facilities open to all include:
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Flow Cytometry at the Department of Pathology
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Flow Cytometry at the Department of Veterinary Medicine
Flow Cytometry at the Department of Pathology
Equipment:
The facility has available not only a few classical flow cytometers (cell sorters and analysers) but also a full spectrum analyser.
Cell Sorters
- Beckman Coulter MoFlo Astrios (5 lasers, 19 detectors)
- BD Aria III (4 lasers, 16 detectors)
- BD Aria IIu (3 lasers, 9 detectors)
Analysers
- CytoFLEX LX (5 lasers, 19 parameters)
- Cytek Biosciences Aurora (5 lasers, full spectrum analyser)
- Thermo Fischer Attune NxT (4 lasers, 14 detectors)
- Beckman Coulter CytoFLEX S (4 lasers, 13 parameters)
- BD LSR II (4 lasers, 17 parameters)
Location
The facility is based in the Department of Pathology (see map)
Contact
Flow Cytometry Facility Manager: Joana Cerveira
Email: flow.cytometry@path.cam.ac.uk
Access and Training
The Flow Cytometry Facility is open to all members of the University and also to commercial users from biotech companies. In house training provided.
More information and booking
Flow Cytometry at the Department of Veterinary Medicine
Equipment
- Zeiss LSM780 Confocal Microscope
- CyAn ADP 9 Colour Analyser
- Imagestream Mark I Imaging Flow Cytometer
Location
The facility is based in the Department of Veterinary Medicine (see map)
Contact
Imaging Facility Manager: Rachel Hewitt
Email: microscopy@vet.cam.ac.uk
Access and Training
The flow cytometry facility is open to all members of the University, external academic institutions and industry. In house training is provided.
More information and booking
