Super-resolution Microscopes
Super-resolution microscopes available in the Platform, including specifications and location:
These are classified as advanced light microscopes for costing purposes.
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PPMS System Name | Zeiss Elyra 7 (SIM & SMLM) | Abberior STED |
Department (Room) | PDN (Anatomy Building, 18) | Genetics (B14A) |
Frame type | inverted | inverted/upright |
System Image |  |  |
Objectives | 10x /0.3 EC Plan-Neofluar DICI, 5.2mm, Dry (Air), 420340-9901-000 | 10x/0.4, UPLSAPO10X2, 3.1mm, Dry (Air) |
40x/1.4, Plan-Apochromat, 0.13mm, Oil, 420762-9900-000 | 20x/0.75, UPLSAPO20X, 0.6mm, Dry (Air) | |
63x/1.4, Plan-Apochromat DICII, 0.19mm, Oil, 420782-9900-799 | 60x/1.2, UPLSAPO60XW, 0.28mm, Water | |
63x/1.46, alpha Plan-Apochromat DICIII, 0.10mm, Oil, 420780-9970-000 | 100x/1.4, UPLSAPO100XO, 0.13mm, Oil | |
Lasers/Lightsource | 405 nm | 4 excitation lasers (405 nm, 488 nm, 561 nm, 640 nm) |
488 nm | 2 STED lasers (775nm & 595nm) | |
561 nm | CoolLED fluorescence excitation (400nm, 490nm, 585nm 635nm) | |
642 nm | ||
Detectors/Cameras | 2x PCO Edge sCMOS cameras | 4x APDs |
Transmitted PMT | ||
Widefield camera | ||
Live Imaging (options) | Yes (Incubation, CO2, Humidity) | Yes |
Z drift compensation | Definite Focus 2, Software based (fluorescence or reflection) | Continuous AF |
Capabilities | Tilescans | 2D and 3D STED capabilities for both colours |
Positions | FLIM detection for confocal and STED imaging | |
3D volumes | Motorised xy scanning stage for tiling and multi-point sampling | |
Lateral resolution (XY): 120 nm | Inverted Microscope Frame (Olympus IX83) | |
Axial resolution (Z): 300 nm | Upright Microscope Frame (Olympus BX63) | |
Acquisition Software | Zen Black | Imspector |