Super-resolution microscopes available in the Platform, including specifications and location:
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PPMS System Name |
Zeiss Elyra 7 (SIM & SMLM) |
Abberior STED |
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Department (Room) |
PDN (Anatomy Building, 18) |
Genetics (B14A) |
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Frame type |
inverted |
inverted/upright |
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System Image |
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Objectives |
10x /0.3 EC Plan-Neofluar DICI, 5.2mm, Dry (Air), 420340-9901-000 |
10x/0.4, UPLSAPO10X2, 3.1mm, Dry (Air) |
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40x/1.4, Plan-Apochromat, 0.13mm, Oil, 420762-9900-000 |
20x/0.75, UPLSAPO20X, 0.6mm, Dry (Air) |
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63x/1.4, Plan-Apochromat DICII, 0.19mm, Oil, 420782-9900-799 |
60x/1.2, UPLSAPO60XW, 0.28mm, Water |
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63x/1.46, alpha Plan-Apochromat DICIII, 0.10mm, Oil, 420780-9970-000 |
100x/1.4, UPLSAPO100XO, 0.13mm, Oil |
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Lasers/Lightsource |
405 nm |
4 excitation lasers (405 nm, 488 nm, 561 nm, 640 nm) |
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488 nm |
2 STED lasers (775nm & 595nm) |
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561 nm |
CoolLED fluorescence excitation (400nm, 490nm, 585nm 635nm) |
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642 nm |
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Detectors/Cameras |
2x PCO Edge sCMOS cameras |
4x APDs |
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Transmitted PMT |
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Widefield camera |
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Live Imaging (options) |
Yes (Incubation, CO2, Humidity) |
Yes |
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Z drift compensation |
Definite Focus 2, Software based (fluorescence or reflection) |
Continuous AF |
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Capabilities |
Tilescans |
2D and 3D STED capabilities for both colours |
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Positions |
FLIM detection for confocal and STED imaging |
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3D volumes |
Motorised xy scanning stage for tiling and multi-point sampling |
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Lateral resolution (XY): 120 nm |
Inverted Microscope Frame (Olympus IX83) |
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Axial resolution (Z): 300 nm |
Upright Microscope Frame (Olympus BX63) |
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Acquisition Software |
Zen Black |
Imspector |
