Confocal Microscopes

Confocal microscopes available in the Platform, including specifications and location:

These are classified as core light microscopes for costing purposes.

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PPMS System NameLeica SP8 ConfocalLeica Stellaris 5 Confocal
Department (Room)Genetics (226)Pharmacology (212)
Frame typeinvertedinverted
 System Image
microscope Leica SP8 confocal on table next to computer
microscope leica stellaris 5 confocal on table next to computer
Objectives10x/0.4 HC PL APO CS2, 2.74mm, Dry (Air), 1150640710x/0.4, HC PL APO CS2, 2.56mm, Dry (Air), 15506424
 20x/0.75, HC PL APO CS2, 0.62mm, Dry (Air) 1150651720x/0.75, HC PL APO CS2, 0.62mm, Dry (Air), 15506517
 40x/1.3, HC PL APO CS2, 0.24mm, Oil, 1150635840x/1.3, HC PL APO CS2, 0.24, Oil, 15506358
 40x/1.1, HC PL APO CS2, 0.65mm, Water, 1150635763x/1.4, HC PL APO CS2, 0.14mm, Oil, 15506350
 63x/1.4, HC PL APO CS2, 0.14mm, Oil, 11506350 
 100x/1.4, HC PL APO CS2, 0.13mm, Oil, 11506372 
Lasers/LightsourceDiode 405 nmDiode 405 nm
 Argon (458, 488 and 514 nm)Diode 488 nm
 DPSS 561 nmWLL (485-685 nm)
 HeNe 594 nm 
 HeNe 633 nm 
Detectors/Cameras2x PMTHyDS x4
 2x HyDTransmitted PMT
 Transmitted PMT 
Live Imaging (options)Yes (Incubation, CO2, Humidity)Yes (Incubation, CO2, Humidity)
Z drift compensationAdaptive Focus Control (AFC), Best FocusBest focus
CapabilitiesResonant scannerZ galvo
 Z galvoTauSense (FLIM-like technique)
 FRAPLightning
 FRAP XTNavigator (tilescans/positions)
 FRET AB3D volumes
 FRET SE 
 Navigator (tilescans/positions) 
 3D volumes 
Acquisition SoftwareLASXLASX

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PPMS System NameZeiss LSM980 Airyscan2 ConfocalZeiss LSM700 Confocal
Department (Room)Biochemistry (Sanger Building 0.14)Pathology (315)
Frame typeinvertedinverted
 System Image
microscope zeiss LSM980 Airyscan2 confocal on table next to computer
microscope zeiss LSM700 confocal on table next to computer
Objectives40x/1.2, C-Apochromat Corr M27, 0.28mm, Water, 421767-9973-799 10x/0.3, EC PlanNeofluar Ph1, 5.2mm, Dry (Air), 420341-9911
 20x/0.8, Plan-Apochromat M27, 0.55mm, Dry (Air), 420650-9903-000 20x/0.5, EC PlanNeofluar Ph2, 2.0mm, Dry (Air), 420341-9904
 40x/1.3, Plan-Apochromat DIC M27, 0.21mm, Oil, 420762-9800-799 40x/1.3, EC PlanNeofluar Ph3, 0.21mm, Oil, 420461-9910
 63x/1.4, Plan-Apochromat, 0.19mm, Oil, 420782-9900-799 63x/1.4, PlanApochromat Ph3, 0.19mm, Oil, 410781-9910
 10x/0.3, EC Plan-Neofluar, 5.2mm, Dry (Air), 420340-9901-000 100x/1.4, PlanApochromat DIC, 0.17mm, Oil, 420792-9900
Lasers/Lightsource405 nmDiode 405 nm
 488 nmDiode 488 nm
 561 nmDiode 555 nm
 639 nmDiode 639 nm
 730 nm 
Detectors/Cameras2x MA-PMT2x PMT
 4x GaAsPTransmitted PMT
 1x NIR-GaAsP 
 1x GaAs 
 1x Airyscan 2 
 Transmitted PMT 
Live Imaging (options)Yes (Incubation, CO2, Humidity)Yes (Incubation)
Z drift compensationDefinite Focus 3, Software based (fluorescence or reflection)Software based (fluorescence or reflection)
CapabilitiesZ-PiezoFRAP
 AI Sample FinderTilescans
 FRAPPositions
 Tilescans3D volumes
 Positions 
 3D volumes 
 Airyscan SR 
 Airyscan Fast 
Acquisition SoftwareZEN Blue 3.6Zen Black

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PPMS System NameZeiss LSM780 Confocal (CL2)Leica SP8 (Core) Confocal
Department (Room)Pathology (314)PDN (Anatomy Building 23)
Frame typeinvertedinverted
 System Image
microscope zeiss LSM780 confocal on table next to computer
microscope Leixa SP8 core confocal
Objectives

10x/0.3, EC Plan-PlanNeofluar Ph1 DICI, 5.2 mm, Dry (Air),

420341-9911-000

10x/0.3, HC PL FLUORTAR DIC, 11mm, Dry (Air), 11506505
 

25x/0.8, Plan-Neofluar Ph2 Imm Korr Ph2 DICIII, 0.41 mm, Multi (Oil/Gly/W),

440545-0000-000

20x/0.75, HC PL APO CS2 DIC, 0.62mm, Dry (Air), 11560517
 40x/1.3, EC Plan-Neofluar Ph3 DICIII, 0.21 mm, Oil, 420461-9910-00025x/0.95, HCX IRAPO L, 2.5mm, Water, 11506323 (Shared)
 63x/1.4, Plan-Apochromat Ph3 DICIII, 0.19 mm, Oil, 420781-9910-00040x/1.3, HC PL APO CS2 DIC, 0.24mm, Oil, 11506358
  40x/1.25, HC PL APO CS2 DIC, 0.35 mm, Glycerol, 11506422 (Shared)
  63x/1.4, HC PL APO CS2 DIC, 0.14mm, Oil, 11506350
Lasers/Lightsource405 nmDiode 405 nm
 Argon (458, 488 and 514 nm)OPSL 488 nm
 561 nmOPSL 552 nm
 633 nmDiode 638 nm
Detectors/Cameras2x PMT2x PMT
 Spectral GaAsP1x HyD
  Transmitted PMT
Live Imaging (options)Yes (Incubation)No
Z drift compensationDefinite Focus, Software based (fluorescence or reflection)Adaptive Focus Control (AFC), Best Focus
CapabilitiesCL2 Live imagingTilescans
 FRAPPositions
 Tilescans3D volumes
 Positions 
 3D volumes 
Acquisition SoftwareZen BlackLASX 3.5

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PPMS System NameLeica SP8 (Advanced) ConfocalZeiss LSM900 Airyscan 2 Confocal
Department (Room)PDN (Anatomy Building 23)PDN (Anatomy Building 22A)
Frame typeinvertedinverted
 System Image
microscope leica SP8 advanced confocal
microscope zeiss LMS900 Airyscan 2 confocal ont table next to computer
Objectives10x/0.4, HC PL APO, 2.2mm Dry (Air), 115062852.5x/0.085, EC Plan-Neofluar, 8.8mm, Dry (Air), 420320-9902-000
 20x/0.75, HC PL APO CS2 DIC, 0.62mm, Dry (Air), 1156051710x/0.45, Plan-Apochromat DICII, 2.0mm, Dry (Air), 420640-9900-000
 25x/0.95, HCX IRAPO L, 2.5mm, Water, 11506323 (Shared)20x/0.8, Plan-Apochromat DICII, 0.55mm, Dry (Air), 420650-9902-000
 40x/1.3, HC PL APO CS2 DIC, 0.24mm, Oil, 1150635840x/1.3, Plan-Apochromat DICIII, 0.21mm, Oil, 420762-9800-000
 40x/1.25, HC PL APO CS2 DIC, 0.35 mm, Glycerol, 11506422 (Shared)63x/1.2, LD LCI Plan-Apochromat Imm Corr DICIII, 0.48mm, Multi (Oil/Gyl), 420882-9870-000
 63x/1.4, HC PL APO CS2 DIC, 0.14mm, Oil, 1150635063x/1.4, Plan-Apochromat DICII, 0.19mm, Oil, 420782-9900-799
Lasers/LightsourceDiode 405 nm405 nm
 Argon (458, 488, 496 and 514 nm)488 nm
 DPSS 561 nm561 nm
 HeNe 594 nm638 nm
 HeNe 633 nm 
Detectors/Cameras2x PMT2x GaSaP
 2x HyD1x Airyscan 2
 Transmitted PMTTransmitted PMT
  Axiocam 503 mono
Live Imaging (options)NoYes (Incubation, CO2, Humidity)
Z drift compensationAdaptive Focus Control (AFC), Best FocusDefinite Focus 2, Software based (fluorescence or reflection)
CapabilitiesResonant scannerTilescans
 FRAPPositions
 Tilescans3D volumes
 PositionsAiryscan SR
 3D volumesAiryscan Fast
Acquisition SoftwareLASX 3.5Zen Blue 3.1

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PPMS System NameLeica Stellaris 5 ConfocalOlympus FV3000 Confocal
Department (Room)Zoology (T13C)Zoology (T13D)
Frame typeuprightinverted
 System Image
microscope Leica Stellaris 5 confocal on table next to computer
microscope Olympus FV3000 confocal
Objectives10x/0.4, 2.56mm, Dry (Air), 115064244x, Dry (Air)
 40x/1.3, 0.24mm, Oil, 1150635810x/0.4, 3.1mm, Dry (Air), UPLSAPO10x2
 63x/0.9, 2.2mm, Water (dipping, no coverglass), 1150614820x/0.75, 0.6mm, Dry (Air), UPLSAPO20x
 40x/0.8, 3.3mm, Water (dipping, no coverglass), 1150615530x/0.95, 0.8mm, Silicon Oil, UPLSAPO30xSilicon
 100x/1.4, 0.09mm, Oil, 1150621060x/1.4, 0.3mm, Silicon Oil, UPLSAPO60xSiicon
 40x/1.25, 0.35mm, Glycerol, 11506422 
 20x/0.7, 0.26-0.17mm, Multi (Oil, glycerol or Water), 11506327 
Lasers/Lightsource405 nm405 nm
 448 nm440 nm
 488 nm488 nm
 514 nm515 nm
 561 nm561 nm
 638 nm594 nm
  640nm
Detectors/Cameras4x Power HyD4x GaAsPs
 Transmitted PMTTransmitted PMT
 Leica K5 sCMOS Monochrome Camera 
Live Imaging (options)NoNo
Z drift compensation Olympus proprietary Z Drift Compensation
CapabilitiesLightningResonant scanner (30 fps at 512 × 512 pixels)
 FRAPMotorized ultra-sonic XY stage
 PhotobleachingBleaching (FRAP) and photo-activation
 FRETZ-drift compensation system
 Tilescans3D deconvolution software
 PositionsDIC optics
 3D Volumes3D Volumes
Acquisition SoftwareLASXFluoView

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