Confocal microscopes available in the Platform, including specifications and location:
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PPMS System Name |
Leica SP8 Confocal |
Leica Stellaris 5 Confocal |
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Department (Room) |
Genetics (226) |
Pharmacology (212) |
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Frame type |
inverted |
inverted |
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System Image |
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Objectives |
10x/0.4 HC PL APO CS2, 2.74mm, Dry (Air), 11506407 |
10x/0.4, HC PL APO CS2, 2.56mm, Dry (Air), 15506424 |
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20x/0.75, HC PL APO CS2, 0.62mm, Dry (Air) 11506517 |
20x/0.75, HC PL APO CS2, 0.62mm, Dry (Air), 15506517 |
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40x/1.3, HC PL APO CS2, 0.24mm, Oil, 11506358 |
40x/1.3, HC PL APO CS2, 0.24, Oil, 15506358 |
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40x/1.1, HC PL APO CS2, 0.65mm, Water, 11506357 |
63x/1.4, HC PL APO CS2, 0.14mm, Oil, 15506350 |
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63x/1.4, HC PL APO CS2, 0.14mm, Oil, 11506350 |
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100x/1.4, HC PL APO CS2, 0.13mm, Oil, 11506372 |
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Lasers/Lightsource |
Diode 405 nm |
Diode 405 nm |
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Argon (458, 488 and 514 nm) |
Diode 488 nm |
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DPSS 561 nm |
WLL (485-685 nm) |
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HeNe 594 nm |
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HeNe 633 nm |
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Detectors/Cameras |
2x PMT |
HyDS x4 |
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2x HyD |
Transmitted PMT |
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Transmitted PMT |
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Live Imaging (options) |
Yes (Incubation, CO2, Humidity) |
Yes (Incubation, CO2, Humidity) |
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Z drift compensation |
Adaptive Focus Control (AFC), Best Focus |
Best focus |
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Capabilities |
Resonant scanner |
Z galvo |
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Z galvo |
TauSense (FLIM-like technique) |
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FRAP |
Lightning |
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FRAP XT |
Navigator (tilescans/positions) |
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FRET AB |
3D volumes |
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FRET SE |
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Navigator (tilescans/positions) |
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3D volumes |
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Acquisition Software |
LASX |
LASX |
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PPMS System Name |
Zeiss LSM980 Airyscan2 Confocal |
Zeiss LSM700 Confocal |
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Department (Room) |
Biochemistry (Sanger Building 0.14) |
Pathology (315) |
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Frame type |
inverted |
inverted |
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System Image |
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Objectives |
40x/1.2, C-Apochromat Corr M27, 0.28mm, Water, 421767-9973-799 |
10x/0.3, EC PlanNeofluar Ph1, 5.2mm, Dry (Air), 420341-9911 |
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20x/0.8, Plan-Apochromat M27, 0.55mm, Dry (Air), 420650-9903-000 |
20x/0.5, EC PlanNeofluar Ph2, 2.0mm, Dry (Air), 420341-9904 |
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40x/1.3, Plan-Apochromat DIC M27, 0.21mm, Oil, 420762-9800-799 |
40x/1.3, EC PlanNeofluar Ph3, 0.21mm, Oil, 420461-9910 |
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63x/1.4, Plan-Apochromat, 0.19mm, Oil, 420782-9900-799 |
63x/1.4, PlanApochromat Ph3, 0.19mm, Oil, 410781-9910 |
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10x/0.3, EC Plan-Neofluar, 5.2mm, Dry (Air), 420340-9901-000 |
100x/1.4, PlanApochromat DIC, 0.17mm, Oil, 420792-9900 |
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Lasers/Lightsource |
405 nm |
Diode 405 nm |
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488 nm |
Diode 488 nm |
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561 nm |
Diode 555 nm |
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639 nm |
Diode 639 nm |
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730 nm |
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Detectors/Cameras |
2x MA-PMT |
2x PMT |
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4x GaAsP |
Transmitted PMT |
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1x NIR-GaAsP |
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1x GaAs |
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1x Airyscan 2 |
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Transmitted PMT |
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Live Imaging (options) |
Yes (Incubation, CO2, Humidity) |
Yes (Incubation) |
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Z drift compensation |
Definite Focus 3, Software based (fluorescence or reflection) |
Software based (fluorescence or reflection) |
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Capabilities |
Z-Piezo |
FRAP |
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AI Sample Finder |
Tilescans |
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FRAP |
Positions |
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Tilescans |
3D volumes |
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Positions |
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3D volumes |
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Airyscan SR |
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Airyscan Fast |
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Acquisition Software |
ZEN Blue 3.6 |
Zen Black |
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PPMS System Name |
Zeiss LSM780 Confocal (CL2) |
Leica SP8 (Core) Confocal |
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Department (Room) |
Pathology (314) |
PDN (Anatomy Building 23) |
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Frame type |
inverted |
inverted |
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System Image |
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Objectives |
10x/0.3, EC Plan-PlanNeofluar Ph1 DICI, 5.2 mm, Dry (Air), 420341-9911-000 |
10x/0.3, HC PL FLUORTAR DIC, 11mm, Dry (Air), 11506505 |
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25x/0.8, Plan-Neofluar Ph2 Imm Korr Ph2 DICIII, 0.41 mm, Multi (Oil/Gly/W), 440545-0000-000 |
20x/0.75, HC PL APO CS2 DIC, 0.62mm, Dry (Air), 11560517 |
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40x/1.3, EC Plan-Neofluar Ph3 DICIII, 0.21 mm, Oil, 420461-9910-000 |
25x/0.95, HCX IRAPO L, 2.5mm, Water, 11506323 (Shared) |
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63x/1.4, Plan-Apochromat Ph3 DICIII, 0.19 mm, Oil, 420781-9910-000 |
40x/1.3, HC PL APO CS2 DIC, 0.24mm, Oil, 11506358 |
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40x/1.25, HC PL APO CS2 DIC, 0.35 mm, Glycerol, 11506422 (Shared) |
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63x/1.4, HC PL APO CS2 DIC, 0.14mm, Oil, 11506350 |
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Lasers/Lightsource |
405 nm |
Diode 405 nm |
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Argon (458, 488 and 514 nm) |
OPSL 488 nm |
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561 nm |
OPSL 552 nm |
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633 nm |
Diode 638 nm |
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Detectors/Cameras |
2x PMT |
2x PMT |
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Spectral GaAsP |
1x HyD |
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Transmitted PMT |
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Live Imaging (options) |
Yes (Incubation) |
No |
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Z drift compensation |
Definite Focus, Software based (fluorescence or reflection) |
Adaptive Focus Control (AFC), Best Focus |
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Capabilities |
CL2 Live imaging |
Tilescans |
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FRAP |
Positions |
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Tilescans |
3D volumes |
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Positions |
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3D volumes |
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Acquisition Software |
Zen Black |
LASX 3.5 |
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PPMS System Name |
Leica SP8 (Advanced) Confocal |
Zeiss LSM900 Airyscan 2 Confocal |
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Department (Room) |
PDN (Anatomy Building 23) |
PDN (Anatomy Building 22A) |
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Frame type |
inverted |
inverted |
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System Image |
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Objectives |
10x/0.4, HC PL APO, 2.2mm Dry (Air), 11506285 |
2.5x/0.085, EC Plan-Neofluar, 8.8mm, Dry (Air), 420320-9902-000 |
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20x/0.75, HC PL APO CS2 DIC, 0.62mm, Dry (Air), 11560517 |
10x/0.45, Plan-Apochromat DICII, 2.0mm, Dry (Air), 420640-9900-000 |
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25x/0.95, HCX IRAPO L, 2.5mm, Water, 11506323 (Shared) |
20x/0.8, Plan-Apochromat DICII, 0.55mm, Dry (Air), 420650-9902-000 |
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40x/1.3, HC PL APO CS2 DIC, 0.24mm, Oil, 11506358 |
40x/1.3, Plan-Apochromat DICIII, 0.21mm, Oil, 420762-9800-000 |
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40x/1.25, HC PL APO CS2 DIC, 0.35 mm, Glycerol, 11506422 (Shared) |
63x/1.2, LD LCI Plan-Apochromat Imm Corr DICIII, 0.48mm, Multi (Oil/Gyl), 420882-9870-000 |
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63x/1.4, HC PL APO CS2 DIC, 0.14mm, Oil, 11506350 |
63x/1.4, Plan-Apochromat DICII, 0.19mm, Oil, 420782-9900-799 |
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Lasers/Lightsource |
Diode 405 nm |
405 nm |
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Argon (458, 488, 496 and 514 nm) |
488 nm |
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DPSS 561 nm |
561 nm |
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HeNe 594 nm |
638 nm |
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HeNe 633 nm |
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Detectors/Cameras |
2x PMT |
2x GaSaP |
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2x HyD |
1x Airyscan 2 |
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Transmitted PMT |
Transmitted PMT |
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Axiocam 503 mono |
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Live Imaging (options) |
No |
Yes (Incubation, CO2, Humidity) |
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Z drift compensation |
Adaptive Focus Control (AFC), Best Focus |
Definite Focus 2, Software based (fluorescence or reflection) |
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Capabilities |
Resonant scanner |
Tilescans |
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FRAP |
Positions |
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Tilescans |
3D volumes |
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Positions |
Airyscan SR |
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3D volumes |
Airyscan Fast |
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Acquisition Software |
LASX 3.5 |
Zen Blue 3.1 |
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PPMS System Name |
Leica SP5 Confocal |
Leica Stellaris 5 Confocal |
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Department (Room) |
Zoology (T13C) |
Zoology (T13C) |
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Frame type |
inverted |
upright |
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System Image |
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Objectives |
10x/0.4, 2.2mm, Dry (Air), 11506285 |
10x/0.4, 2.56mm, Dry (Air), 11506424 |
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20x/0.7, 0.26-0.17mm, Multi (Oil/Gly/W), 11506191 |
40x/1.3, 0.24mm, Oil, 11506358 |
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40x/1.25, 0.1mm, Oil, 11506253 |
63x/0.9, 2.2mm, Water (dipping, no coverglass), 11506148 |
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63x/1.4, 0.1mm, Oil, 11506192 |
40x/0.8, 3.3mm, Water (dipping, no coverglass), 11506155 |
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63x/1.2, 0.22mm, Water, 11506280 |
100x/1.4, 0.09mm, Oil, 11506210 |
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40x/1.25, 0.35mm, Glycerol, 11506422 |
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20x/0.7, 0.26-0.17mm, Multi (Oil, glycerol or Water), 11506327 |
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Lasers/Lightsource |
405 nm |
405 nm |
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Argon (458, 476, 488, 496 and 514 nm) |
448 nm |
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594 nm |
488 nm |
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561 nm |
514 nm |
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633 nm |
561 nm |
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638 nm |
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Detectors/Cameras |
3x PMT |
4x Power HyD |
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2x HyD |
Transmitted PMT |
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Transmitted PMT |
Leica K5 sCMOS Monochrome Camera |
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Live Imaging (options) |
Yes (Incubation, CO2, Humidity) |
No |
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Z drift compensation |
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Capabilities |
Resonant scanner |
Lightning |
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Tilescans |
FRAP |
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Positions |
Photobleaching |
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3D volumes |
FRET |
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DIC optics |
Tilescans |
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Positions |
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3D Volumes |
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Acquisition Software |
LAS AF |
LASX |
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PPMS System Name |
Olympus FV3000 Confocal |
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Department (Room) |
Zoology (T13D) |
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Frame type |
inverted |
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System Image |
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Objectives |
4x, Dry (Air) |
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10x/0.4, 3.1mm, Dry (Air), UPLSAPO10x2 |
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20x/0.75, 0.6mm, Dry (Air), UPLSAPO20x |
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30x/0.95, 0.8mm, Silicon Oil, UPLSAPO30xSilicon |
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60x/1.4, 0.3mm, Silicon Oil, UPLSAPO60xSiicon |
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Lasers/Lightsource |
405 nm |
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440 nm |
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488 nm |
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515 nm |
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561 nm |
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594 nm |
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640nm |
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Detectors/Cameras |
4x GaAsPs |
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Transmitted PMT |
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Live Imaging (options) |
No |
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Z drift compensation |
Olympus proprietary Z Drift Compensation |
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Capabilities |
Resonant scanner (30 fps at 512 × 512 pixels) |
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Motorized ultra-sonic XY stage |
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Bleaching (FRAP) and photo-activation |
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Z-drift compensation system |
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3D deconvolution software |
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DIC optics |
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3D Volumes |
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Acquisition Software |
FluoView |
