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School of the Biological Sciences

Spinning Disk Confocal microscopes in the Platform, including specifications and location:

These are classified as core light microscopes for costing purposes.

 

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PPMS System Name

Nikon W1-SoRa

Evident SpinSR

Department (Room)

PDN (Anatomy Building 22A)

Pathology (314)

Frame type

inverted

inverted

 System Image

 


 

Objectives

4x/0.2NA, CFI Plan Apochromat Lambda D, 20 mm, Air, MRD70040

4x/0.16NA, UPLXAPO4X, 13 mm, Air

 

10x/0.45NA, CFI Plan Apochromat Lambda D, 4 mm, Air, MRD70170

10x/0.4NA, UPLXAPO10X, 3.1 mm, Air

 

20x/0.8NA, CFI Plan Apochromat Lambda D, 0.8 mm, Air, MRD70270

20x/0.8NA, UPLXAPO20X, 0.6 mm, Air

 

25x/1.05NA, CFI Plan Apo Lambda S, 0.55 mm, Silicone Oil, MRD73250

30x/1.05NA, UPLSAPO30XS, 0.8 mm, Silicone

 

40x/1.25NA, CFI Plan Apo Lambda S, 0.3 mm, Silicone Oil, MRD73400

60x/1.3NA, UPLSAPO60XS2, 0.3 mm, Silicone

 

60x/1.42NA, CFI Plan Apochromat Lambda D, 0.15 mm, Oil, MRD71670

40x/1.4NA, UPLXAPO40XO, 0.13 mm, Oil

 

 

60x/1.5NA, UPLAPO60XOHR, 0.11 mm, Oil

Lasers/Lightsource

405 nm

405 nm

 

488 nm

488 nm

 

561 nm

561 nm

 

638 nm

594 nm

 

 

640 nm

Detectors/Cameras

2x Teledyne Photometrics Kinetix, 2400 x 2400, 6.5 µm pixels

2x Hamamatsu BT-Fusion, 2333 x 2333, 6.5 µm pixels

Live Imaging (options)

Yes (Incubation, CO2, Humidity)

Yes (Incubation, CO2, Humidity)

Z drift compensation

Perfect Focus System (PFS)

Z Drift Compensation (ZDC)

Capabilities

Photomanipulation 405 and 488 nm

Photomanipulation 405 nm

 

Widefield bypass

Widefield bypass

 

Yokogawa CSU-W1 SoRa 50 µm confocal disk, 50 µm SoRa disk

(Mag changer 1x, 2.8, 4x), 4000 rpm

Yokogawa CSU-W1 SoRa 50 µm confocal disk, 50 µm SoRa disk

(Mag changer 1x, 3.2x), 4000 rpm

 

MCL Piezo Z, range 500 µm

MCL Piezo Z, range 300 µm

 

Tilescans

Tilescans

 

Multi positions

Multi positions

 

Live imaging

Live imaging

 

3D Volumes

3D Volumes

Acquisition Software

NIS Elements, JOBS, General Analysis, 2D/3D Deconvolution

cellSens, Wellplate navigator