Dr. Hui Hong

Wellcome Trust post–doc Research Associate (2006–present), Department of Biochemistry, University of Cambridge;
 Research Associate (2001–2005), Department of Chemistry,
University of Cambridge;
Ph. D (1992–1997) in Analystical and Environmental Chemsitry, Nanjing University,Nanjing, P. R. China

hh230@cam.ac.uk
 
 
 
 
 
 
   
  Research Interests

Application of mass spectrometry in the study of polyketide natural products and their biosynthesis.

 

Publications

  1. L. Smith, H. Hong, J.B. Spencer, P. F. Leadlay. Analysis of specific mutants in the lasalocid gene cluster: evidence for enzymatic catalysis of a disfavoured polyether ring closure. (2008) ChemBioChem, 9 (18):2967–75.
  2. D.H. Kwan, Y. Sun, F. Schulz, H. Hong, B. Popovic, J.C. Sim-Stark, S.F. Haydock, P. F. Leadlay. Prediction and manipulation of the stereochemistry of enoylreduction in modular polyketide synthases. (2008) Chem Biol., 15 (11):1231–40.
  3. H. Hong, E. Coutanceau, M. Leclerc, L. Caleechurn, P. F. Leadlay, C. Demangel. Mycolactone Diffuses from Mycobacterium ulcerans–Infected Tissues and Targets Mononuclear Cells in Peripheral Blood and Lymphoid Organs. (2008) PLoS Negl Trop Dis., 2 (10):e325.
  4. H. Hong, D. Spiteller, J. B. Spencer. Incorporation of fluoroacetate into an aromatic polyketide and its influence on the mode of cyclization. (2008) Angew Chem Int Ed Engl., 47 (32):6028–32.
  5. H. Hong, C. Demangel, S. J. Pidot, P. F. Leadlay, T. Stinear. Mycolactones: immunosuppressive and cytotoxic polyketides produced by aquatic mycobacteria. (2008) Nat Prod Rep., 25 (3): 447–54.
  6. S. Pidot, H. Hong, T. Seemann, J. Porter, M. Yip, P. Wilson, A. Men, M. Johnson, J. Davies, P. Leadlay, T. Stinear. Deciphering the genetic basis for polyketide variation among mycobacteria producing mycolactones. (2008) BMC Genomics, 9(1): 462.
  7. Y. Demydchuk, Y. H. Sun, H. Hong, J. Staunton, J. B. Spencer, P. F. Leadlay. Analysis of the tetronomycin gene cluster: insights into the biosynthesis of a polyether tetronate antibiotic. (2008) ChemBioChem, 9 (7):1136–45.
  8. Y. H. Sun, H. Hong, F.Gillies, J. B. Spencer, P. F. Leadlay. Glyceryl–S–acyl carrier protein as an intermediate in the biosynthesis of tetronate antibiotics. (2008) ChemBioChem, 9 (1):150–6.
  9. H. Hong, T. Stinear, J. Porter, C. Demangel, P. F. Leadlay. A novel mycolactone toxin obtained by biosynthetic engineering. (2007) ChemBiocChem, 8 (17):2043–7.
  10. B. M. Harvey, T. Mironenko, Y. H. Sun, H. Hong, Z. X. Deng, P. F. Leadlay, K. J. Weissman, S. F. Haydock. Insights into polyether biosynthesis from analysis of the nigericin biosynthetic gene cluster in Streptomyces sp. DSM4137. (2007) Chem Biol., 14 (6):703–14.
  11. Y. H. Sun, H. Hong, M. Sarnboskyy, T. Mironenko, P. F. Leadlay, S. F. Haydock. Organization of the biosynthetic gene cluster in Streptomyces sp. DSM 4137 for the novel neuroprotectant polyketide meridamycin. (2006) Microbiology, 152 (12):3507–15.
  12. B. M. Harvey, H. Hong, M. A. Jones, Z. A. Hughes–Thomas, R. M Goss, M. L. Heathcote, V. M. Bolanos–Garcia, W. Kroutil, J. Staunton, P. F. Leadlay, J. B. Spencer. Evidence that a novel thioesterase is responsible for polyketide chain release during biosynthesis of the polyether ionophore monensin. (2006) ChemBioChem, 7 (9):1435–42.
  13. M. A. Gregory, H. Hong, R. E. Lill, S. Gaisser, H. Petkovic, L Low, L. S. Sheehan, I. Carletti, S. J. Ready, A. L. Kaja, A. J. Weston, I. R. Challis, M. J. Ward, P. F. Leadlay, C. J. Martin, B. Wilkinson, R. M. Sheridan. Rapamycin biosynthesis: elucidation of gene product function. (2006) Organic & Biomolecular Chemistry, 4 (19): 3565–3568.
  14. K. J. Weissman, H. Hong, B. Popovic, F. Meersman. Evidence for a protein–protein interaction motif on an acyl carrier protein domain from a modular polyketide synthase. (2006) Chem Biol., 13 (6):625–36.
  15. H. Hong, T. Stinear, P. Skelton, J. B. Spencer, P. F. Leadlay. Structure elucidation of a novel family of mycolactone toxins from the frog pathogen Mycobacterium sp. MU128FXT by mass spectrometry. (2005) Chem Commun, 14 (34):4306–8.
  16. H. Hong, A. N. Appleyard, A. P. Siskos, J. Garcia–Bernardo, P. F. Leadlay, J. Staunton. Chain initiation on Type I Modular Polyketide Synthases revealed by Limited Proteolysis and Ion–trap Mass Spectrometry (2005) Euro. J. Biochem., 272 (10): 2373–2387.
  17. H. Hong, J. B.Spencer, J. L. Porter, P. F. Leadlay, T. Stinear. A Novel Mycolactone from a Clinical Isolate of Mycobacterium ulcerans Provides Evidence for Additional Toxin Heterogeneity as a Result of Specific Changes in the Modular Polyketide Synthase. (2005) ChemBioChem 6 (4): 643–648.
  18. T. Stinear, H. Hong, W. Frigui, M. J. Pryor, R. Brosch, T. Garnier, P. F. Leadlay, S. T. Cole. A common evolutionary origin for the unstable virulence plasmid pMUM found in diverse strains of Mycobacterium ulcerans. (2004) J. Bacteriol. 182 (22): 6322–6330.
  19. K. J. Weissman, H. Hong, M. Oliynyk, A. P. Siskos, , P. F. Leadlay. Identification of a phosphopantetheinyl transferase for erythromycin biosynthesis in Saccharopolyspora erythraea. (2004) Chembiochem, 5:116–125.
  20. M. A.Gregory, S. Gaisser, R. E. Lill, H. Hong, M. S. Rose, B. Wilkinson, H. Petkovic, A. J.Weston, I. Carletti, H. L. Lee, J. Staunton, P. F. Leadlay. Isolation and Characterization of Pre–rapamycin, the First Macrocyclic Intermediate in the Biosynthesis of the Immunosuppressant Rapamycin by S. hygroscopicus. (2004) Angew. Chem. Int. Ed. Engl. 43 (19): 2551–2553.
  21. H. Hong, P. J. Gates, J. Staunton, T. Stinear, S. T. Cole, P. F. Leadlay, J. B. Spencer. Identification using LC–MSn of co–metabolites in the biosynthesis of the polyketide toxin mycolactone by a clinical isolate of Mcobacterium ulcerans. (2003) Chem. Commun., 2822–2823.
  22. J. Bernardo–Garcia, L. Xiang, H. Hong, B. S. Moore, P. F. Leadlay. Phenyl–substituted Polyketides produced by Precursor-directed Biosynthesis. (2004) ChemBioChem. 5 (8): 1129–1131.
  23. C. M. Squire, R. J. M. Goss, H. Hong, P. F. Leadlay, J. Staunton. Catalytically active tetramodular 6–deoxyerythonolide B synthase fusion proteins. (2003) ChemBioChem 4 (11): 1225–1228.
  24. A. Crotti, T. Fonseca, H. Hong, S. Galembeck, J. Staunton, N. Lopes, P. Gates. Fragmentation Mechanisms of Five Membered Lactones by Electrospray Ionisation Mass Spectrometry. International Journal of Mass Spectrometry, 232, 271–276.
  25. N. P. Lopes, C. B. W. Stark, H. Hong, P. J. Gates and J. Staunton Structural Elucidation Studies on Monensin A and B by Accurate–Mass Electrospray Tandem Mass Spectrometry. (2002) Rapid Communications in Mass Spectrometry 16 (5): 414–420.
  26. N. P. Lopes, C. B. W. Stark, H. Hong, P. Gates, J. Staunton. A study of the effect of pH, solvent system, cone potential and the addition of crown ethers on the formation of the monensin protonated parent ion in electrospray mass spectrometry. (2001) Analyst, 126, 1630–1632.
  27. T. L. Li, O. W. Choroba, H. Hong, D. H. Williams, J. B. Spencer. Biosynthesis of the Vancomycin Group of Antibiotics: Characterisation of a type III polyketide synthase in the Pathway to (S)–3,5–Dihydroxyphenylglycine. (2001). Chem. Commun., 20, 2156–2157.
  28. H. Hong, S. K. Han,. Prediction of Partition Coefficient and Toxicity for Phenylthio, Phenylsulfinyl and Phenylsulfonyl Acetates. (1995) Environ. Sci. Technol. 29 (12), 3044–3048.
  29. H. Hong, D. Zhou, S. K. Han L. S. Wang. Retention of Aromatic Sulfur-containing Compounds on RP–HPLC: Correlation with Partition Coefficient and Molecular Connectivity Indices. (1996) J. Liq. Chrom. & Rel. Technol. 19 (10), 1617–1629.
  30. H. Hong, L. S. Wang, S. K. Han. The Estimation of Bioconcentration Factors of Aromatic Hydrocarbons by High Performance Liquid Chromatography. (1996) Toxicol. Environ. Chem, 56, 185–191.

 
   
   
 
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