Illumina MiSeq
Information courtesy of Illumina
Introduction
The Illumina MiSeq System combines proven sequencing by synthesis (SBS) technology with a workflow that enables you to go from DNA to analyzed data in as little as eight hours. The MiSeq integrates cluster amplification, sequencing and data analysis in a single instrument.
| MiSeq Performance |
|---|
|
Total time |
Output |
| Read Length |
Current |
Current |
| 2 x 25 bp |
5.5 hrs |
750 - 850 Mb |
| 2 x 150 bp |
24 hrs |
4.5 - 5.1 Gb |
| 2 x 250 bp |
>39 hrs |
7.5 - 8.5 Gb |
| Reads Passing Filter |
Current |
| Single Reads |
15 - 17 M |
| Paired-End Reads |
30 - 34 M |
| Quality Scores |
| 2 x 25 bp |
> 90% bases higher than Q30 |
| 2 x 150 bp |
> 80% bases higher than Q30 |
| 2 x 250 bp |
> 70% bases higher than Q30 |
Applications
The MiSeq supports a wide range of applications, including the following:
- Targeted resequencing
- TruSeq Custom Amplicon Sequencing
- De novo sequencing (< 20Mb for ∼ 50x coverage, < 50Mb for ∼ 20x coverage)
- Small RNA sequencing
- Metagenomics
- Library QC
Library Preparation
You can sequence libraries prepared by any of the following Illumina sample preparation kits:
- TruSeq DNA sample prep kit
- TruSeq RNA sample prep kit
- TruSeq Small RNA sample prep kit
- TruSeq Custom Amplicon kit
- Nextera Sample prep kit
- NexteraXT Sample prep kit
To submit a sample for Illumina MiSeq Sequencing
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