Illumina MiSeq
Information courtesy of Illumina
Introduction
The Illumina MiSeq System combines proven sequencing by synthesis (SBS) technology with a workflow that enables you to go from DNA to analyzed data in as little as eight hours. The MiSeq integrates cluster amplification, sequencing and data analysis in a single instrument.
Performance
- Output - > 1 Gb
- Single Reads - 3.4 Million
- Paired-end Reads - 6.8 Million
- Required sample input - 50 ng with Nextera, 100 ng - 1 Μg with TruSeq
- Read Length - 2 x 150 bp
- Percentage of Bases > Q30 - > 85% (2 x 50 bp), > 80% (2 x 100 bp)
Applications
The MiSeq supports a wide range of applications, including the following:
- Targeted resequencing
- TruSeq Custom Amplicon Sequencing
- De novo sequencing (< 20Mb for ∼ 50x coverage, < 50Mb for ∼ 20x coverage)
- Small RNA sequencing
- Metagenomics
- Library QC
Library Preparation
You can sequence libraries prepared by any of the following Illumina sample preparation kits:
- TruSeq DNA sample prep kit
- TruSeq RNA sample prep kit
- TruSeq Small RNA sample prep kit
- TruSeq Custom Amplicon kit
- Nextera Sample prep kit
To submit a sample for Illumina MiSeq Sequencing
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