| Consideration of multicolour fluorochrome combinations which should work well on the Cytek DxP8 | ||||||||||||
| SIX COLOUR | FIVE COLOUR | SEVEN COLOUR | ||||||||||
| 1st choice | alternatives | less good | ||||||||||
| BluFL1 | alexa488 | FITC | alexa488 | alexa488 | alexa488 | |||||||
| BluFL2 | (PE) | (PE) | (PE) | (PE) | ||||||||
| BluFL3 | PerCP-Cy5.5 | PE-Cy5.5 | PerCP | PerCP-Cy5.5 | PerCP-Cy5.5 | PerCP-Cy5.5 | ||||||
| BluFL4 | PE-Cy7 | |||||||||||
| RedFL1 | eFluor660 | alexa 660 | alexa647 | eFluor660 | eFluor660 | eFluor660 | ||||||
| RedFL2 | APC-eFluor780 | APC-alexa 750 | APC-eFluor780 | APC-eFluor780 | ||||||||
| YellowFL1 | PE | PE | PE | PE | ||||||||
| YellowFL2 | Texas Red | Texas Red | Texas Red | |||||||||
| notes | 1 | 2 | ||||||||||
| 1 | This 6 colour stain will work best if the weaker antigens are assigned to the PE and efluor660 channels and the stronger to Texas Red and APC-eFluor780 | |||||||||||
| 2 | alternatively turn the yellow laser off and use BLUFL2 for PE | |||||||||||
| some fluorochromes will take up 2 channels e.g PE - bold shows the 'strongest' (theoretical); you cannot compensate a fluorochrome against itself, it might be best to 'turn off' one PMT in some circumstances | ||||||||||||
| PE | BLUFL2 | YellowFL1 | ||||||||||
| alexa 568 | Yellow FL1 | YellowFL2 | ||||||||||
| PE-Cy5 | Blu FL3 | Red FL1 | ||||||||||
| PI | Blu FL2 | BluFL3 | yellowFL1 | YellowFL2 | ||||||||
| PI is a special case; you probably can gate out the dead cells and still use some spillover channels | ||||||||||||
| The above suggestions are not intended to be an exhaustive list of fluorochromes which will work. Ask Nick or Nigel if you want further information | ||||||||||||